sigB [2017-8-11 16:0:3]

[SW|RNA polymerase] [SW|sigma factor] SigB

locus: BSU04730

pI: 5.42

mw: 29.99 kDa

protein length: 264 aa

gene length: 792 bp

function: general stress response

product: [SW|RNA polymerase] [SW|sigma factor] SigB

essential: no

synonyms: rpoF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]

Gene

Coordinates: 522,862 → 523,650

The protein

Protein family

• SigB subfamily (according to Swiss-Prot)

Structure

• [PDB|1L0O] ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]-[protein|search|SpoIIAB ]complex from Geobacillus stearothermophilus, 32% identity) [pubmed|11955433]

Expression and Regulation

Operons

• genes: [gene|621357D1FB6AC1B0499732EFE226F982B26CE34E|rsbR]-[gene|841CE7FCA4E84445830CA18F9856F6F30014E3BB|rsbS]-[gene|ADC52A22950736A0435AEEEC43F7407878786A81|rsbT]-[gene|0B8381D2C724DAA724E4AFC67000F12AD112D13D|rsbU]-[gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
  description: [Pubmed|8002610]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8002610], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|20454630], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|AC64DA463250A090A62E50901EFE653C8F963872|rsbV]-[gene|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|rsbW]-[gene|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-[gene|8536803BDFB58D43C52961931F609933E49E989D|rsbX]
  description: [Pubmed|2170324]

  regulation

  • ''[protein|search|rsbV]:'' induced by stress ([protein|search|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• QB5344 (cat), available in the [SW|Stülke] lab
• 1A675 ( ''sigB''::''cat''), [Pubmed|3027048], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A675&Search=1A675 BGSC]
• 1A777 ( ''sigB''::''cat''), [Pubmed|1744042], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A777&Search=1A777 BGSC]
• 1A780 ( ''sigB''::''spec''), [Pubmed|8253681], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A780&Search=1A780 BGSC]

Labs working on this gene/protein

• [SW|Bill Haldenwang], San Antonio, USA
• [SW|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]

References

Reviews

• 19575568,18035607,11407115,9767581,20658979,27518094,28271471

Control of SigB activity by protein-protein interactions

• 11377871,12270815,15312768,8460143,8682769,8002610,11591687,7601843,17498739,15342582

Identification of the SigB regulon

• 11544224,11532142,11717291,10482513,10220166,22174379

Other publications

• 23407164,22511268,22210769,23524614,21979936,8655572,3123466,10369900,3112122,8468294,8458834,13129942,6784117,12867438,3100810,8253681,12486038,8764398,3027048,15342585,17575448,17586624,23934352,25652417,3016731,11902719,14651641,10503549,15205443,6405278,10383961,15805528,6790515,116131,39767581,19948797,22582280,24572018,24878601,27977677,28461450,11955433,28727759